• Culture plate areas and number of fibroblasts for confluence (.doc)

• Antibodies for flow cytometry (.xls)

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• Svedberg-to-kilodalton estimation (.xls)

• Bradford assay protein concentration calculator (.xls)

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• 5' end phosphorylation (.doc)

• Covalent crosslinking of proteins to protein A separose beads (.doc)

• CsCl preps (.doc)

• Deriving mouse macrophages from fetal liver (.doc)

• Electrotransformation of DNA into bacteria and preparation of electrocompetent cells (.doc)

• Fusion Protocol (for making hybridomas) (.doc)

• Immunohistochemistry (paraffin sections) (.doc)

• In vitro transcription using the Gless casette (.doc)

• Immunostaining (.doc)

• Infection of fetal liver hematopoietic stem cells with lentivirus for adoptive transfer (.doc)

• Minipreps - old school (.doc)

• Ni column recharge (.doc)

• Producing retroviruses (.doc)

• Purification of GST fusion proteins (.doc)

• Stripping and reprobing Western and Southern blots (.doc)

• Run-off transcription (.doc)

• UV crosslinking (.doc)

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• Dean's recipies (.doc)